Endocrine Abstracts

Endothelial cells (ECs) have major role in post-ischemic angiogenesis. Trimethylation of lysine 27 of histone3 (H3K27me3) is a repressive epigenetic mark carried by EZH2 enzyme, the catalytic part of Polycomb complex2 (PRC2, also comprising the suppressor of zeste 12 homolog (Suz12)). We investigated if in vitro hypoxia and in vivo limb ischemia affect the expression of PRC2 in ECs and mouse limb muscles, respectively. Then, modulation of angiogenic genes by ...

Objectives: Endothelial cells (ECs) have major role in post-ischemic angiogenesis. EZH2 (enhancer of zest homology) carries out trimethylation of lysine 27 on histone3 (H3K27me3) – repressive mark, thus modulating gene expression. We tested if the EZH2 inhibitor 3-deazaneplanocin (DZNep) regulates angiogenesis under hypoxia and limb ischemia (LI) in vivo in ECs and mouse limb muscles respectively.Methods: Human umbilical vein ECs...

Endothelial cells (ECs) may display distorted epigenetic features in diabetes mellitus (DM) but this has not been investigated under combined conditions of diabetes and limb ischemia. Initial screening for histone H3 and H4 trimethylation (me3) and panacetylation of lysine residues (causing transcriptional repression and activation) were conducted using two models of hyperglycemia and ischemia. Human umbilical vein ECs (HUVECs) were cultured in EGM2 or EBM2 media (low growth f...